Assembly Output

Annotations and contigs are found in the top-level of the output. The contigs represent the passing contigs after filtering has been performed. The annotations table contains metrics on the contigs, open reading frames, genomics bins, taxonomy assignments, other functional annotations, and the coverage count across the genomic regions.

Sequence Quality Control

Paired-end and single-end reads with 00 have been quality trimmed and filtered of adapter sequences using BBDuk2. The minimum length threshold and sequence complexity filters are also applied to these reads.

Paired-end reads with 01 represent error corrected reads using Tadpole of the BBTools package.

FASTQ files with 02 represent sequences pulled from 01, or 00 if no error correction has been performed, that are hits to the specified contamination reference database defined in the file name.

Sequence reads in 03 represent reads after decontamination where if the sample is a metagenome, rRNA reads have been added back into the pool. rRNA reads are not added back for metatranscriptomes.

Reads that have normalized coverage across kmers are in 04.

Assembly

Unfiltered contigs and stats associated with the assembly, coverage across contigs before and after filtering, and logs associated with these steps.

Prefiltered contigs represents unfiltered contigs immediately following assembly.

Discarded contigs represents a subset of contigs present in prefiltered contigs that were omitted from the final contigs due to coverage or length parameters set by the user.

Sequence Alignment

Holds temporary alignment files as well as the final alignment file after Picard MarkDuplicates has been run against the alignments. This file is used in the quantification step by featureCounts.

Annotation

Holds functional annotation output and raw counts across functional regions.

Feature Counts

Per loci read counts and a summary of read mapping for the sample.

Prokka

All Prokka output including the amino acid sequence FASTA and associated functional annotation. The “plus” file represents a fixed TSV format that ensures all rows have the same number of columns.

RefSeq

Includes raw BLAST hits, sorted BLAST hits with an added first column which enables contig to open reading frame mapping, and taxonomy assignments per loci.

Genomic Bins

Contains the bins determined by MaxBin2 and all checkm output including its taxonomy assignments and completeness estimates per bin. These metrics are combined into a single table in the top level of the sample output directory.

Reference Genome (ref)

Contains a concatenated genome of contamination references and will be used on subsequent samples being output to the same results output directory.